Journal: Nature Plants

Article Title: Targeted base editing in the plastid genome of Arabidopsis thaliana

doi: 10.1038/s41477-021-00954-6

Figure Lengend Snippet: Artificially synthesized CD half coding sequence (Supplementary Table ) and entry vectors for step2 assembly containing FokI coding sequence were amplified with primes corresponding to the template (Supplementary Table ). The purified PCR products were mixed with 5× In-Fusion HD Cloning Enzyme Premix (TaKaRa) and incubated at 50 °C for 15 minutes.

Article Snippet: The assembled destination vector was digested by KpnI and the purified product was incubated at 50 °C for 15 minutes after mixed with 5× In-Fusion HD Cloning Enzyme Premix and OLE1-GFP coding sequence amplified from pFAST02 (INPLANTA INNOVATIONS INC), to make the destination vector for ptpTALECD expression vector construction.

Techniques: Synthesized, Sequencing, Amplification, Purification, Clone Assay, Incubation

Journal: Nature Plants

Article Title: Targeted base editing in the plastid genome of Arabidopsis thaliana

doi: 10.1038/s41477-021-00954-6

Figure Lengend Snippet: a , The 2 nd entry vector construction. The 2 nd entry vector used in Arimura et al. , and RECA1 plastid transit peptide coding sequence (PTP; Supplementary Table ) were amplified with primers corresponding to the DNA template (Supplementary Table ). The purified PCR products (1: PTP + 2 nd entry vector, 2: PTP + destination vector) were mixed with 5× In-Fusion HD Cloning Enzyme Premix (TaKaRa) and incubated at 50 °C for 15 minutes. b , The destination vector construction. The destination vector used in Arimura et al . (2020) was amplified with primers (Supplementary Table ); purified PCR product was mixed with the PTP PCR product and 5× In-Fusion HD Cloning Enzyme Premix and incubated at 50 °C for 15 minutes to make the destination vector for PTP-GFP expression vector construction. The assembled destination vector was digested by KpnI and the purified product was incubated at 50 °C for 15 minutes after mixed with 5× In-Fusion HD Cloning Enzyme Premix and OLE1-GFP coding sequence amplified from pFAST02 (INPLANTA INNOVATIONS INC), to make the destination vector for ptpTALECD expression vector construction.

Article Snippet: The assembled destination vector was digested by KpnI and the purified product was incubated at 50 °C for 15 minutes after mixed with 5× In-Fusion HD Cloning Enzyme Premix and OLE1-GFP coding sequence amplified from pFAST02 (INPLANTA INNOVATIONS INC), to make the destination vector for ptpTALECD expression vector construction.

Techniques: Plasmid Preparation, Sequencing, Amplification, Purification, Clone Assay, Incubation, Expressing